| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 08 Lady Lobos Rush Premier |
0603-011G4U-0011 | Candice Spiniolas |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 08G |
0324-021G4U-0027 | Daniel McBride |
| A3 | Knoxville FC (Crush) Knoxville FC Crush 08 G Premier |
0206-011G4U-0035 | Lindsay Taylor |
| A4 | Knoxville Reds Football Club - 01 Knoxville Reds 08 Newcastle |
0290-01D2G4U-0010 | Josiah Strain |
| A5 | Kings Hammer MSC 08 Lady Strikers Showcase |
0515-01AG4U-0120 | Alan Oates |
| A6 | Nashville United Soccer Academy - 01 NUSA G08 Maroon |
0534-011G4U-0002 | Adam Harris |
| A7 | Tennessee Soccer Club TSC 2008 Girls Nike |
0522-011G4U-0054 | Lance Mayes |
| A8 | Tennessee United Soccer Club TUSC 2008 Girls SCCL |
0424-011G4U-0003 | Miguel Arriaga |
| A9 | USA North USA North 08G |
0218-01D1G4U-0408 | Darrick Lubell |
| A10 | Wilson United Soccer Club Wilson United - G14U |
0406-012G4U-0003 | Mike Moore |