| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Blount United Soccer Club Blount United Soccer Club - G13U |
0201-012G3U-0240 | Darren Sutton |
| A2 | Chattanooga FC Academy Chattanooga FC Academy - 2009G White |
0316-024G3U-7845 | Gary Smith |
| A3 | Gallatin Rangers GSC Lady Rangers 09 |
0408-023G3U-0049 | Michael Thomas |
| A4 | Knoxville FC (Crush) Knoxville FC Crush 09 G Elite |
0206-013G3U-0132 | Victor Flores |
| A5 | Knoxville Reds Football Club - 01 Knoxville Reds 10 Roma |
0290-01SJG2U-0008 | Julia French |