| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 09 Lady Lobos Rush Elite |
0603-012G3U-0021 | Candice Spiniolas |
| A2 | Lobos Rush (Collierville Soccer Association) 10 Lady Lobos Rush Blue |
0603-01SG2U-0008 | John Cooke |
| A3 | Clarksville Soccer Club Clarksville SC - G2009 Maroon |
0422-012G3U-0007 | Malick Sakande |
| A4 | Nashville United Soccer Academy - 01 NUSA G09 Navy |
0534-012G3U-0054 | Jill Condon |
| A5 | Soccer Ole OLE FC 09G Premier |
0632-022G3U-0005 | Robby Midyett |
| A6 | Tennessee Soccer Club TSC 2010 Girls Showcase White |
0522-01AG2U-2063 | Dean Blain |
| A7 | Tennessee Soccer Club TSC Williamson 2009 Girls Showcase |
0522-012G3U-0024 | Jenger Parrish |