| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Knoxville FC (Crush) Knoxville FC Crush 09 G Premier |
0206-012G3U-0121 | Paige Huffaker |
| A2 | Kings Hammer MSC 09 Lady Strikers Showcase |
0515-01AG3U-0121 | Alan Oates |
| A3 | Tri-Cities United Soccer Club Tri-Cities United 09 Girls Premier |
0106-012G3U-0001 | Malora Quesinberry |
| A4 | Tennessee United Soccer Club TUSC 2009 Girls Red |
0424-012G3U-0002 | Sykia Perry |
| A5 | Tennessee United Soccer Club TUSC 2010 Girls Red |
0424-01AG2U-0040 | Donnie Evans |
| A6 | Wilson United Soccer Club Wilson United - G13U |
0406-012G3U-0002 | Weber Costa |