| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 09 Lady Lobos Rush Premier |
0603-011G3U-0029 | Julia Osmond |
| A2 | Armada Futbol Club Armada FC 09G Red |
0436-013G3U-0002 | Mark Champion |
| A3 | Chattanooga FC Academy Chattanooga FC Academy - 2009G Navy |
0316-02GG3U-7844 | Stephen Hirayama |
| A4 | Knoxville Reds Football Club - 01 Knoxville Reds 09 Ajax |
0290-01D1G3U-0002 | Derrick Long |
| A5 | Nashville United Soccer Academy - 01 NUSA G09 Maroon |
0534-011G3U-0001 | Cameron Eades |
| A6 | Tennessee Soccer Club TSC 2009 Girls Nike |
0522-011G3U-0104 | Lee Smith |
| A7 | Tennessee Soccer Club TSC 2010 Girls Showcase Blue |
0522-011G3U-2069 | Tre Arhagba |
| A8 | Tennessee United Soccer Club TUSC 2009 Girls SCCL |
0424-011G3U-0001 | Andrew Fish |