| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy - 2011G Navy |
0316-029G1U-7848 | James Winecoff |
| A2 | Knoxville FC (Crush) Knoxville FC Crush 2010G Dortmund |
0206-01CG2U-5556 | Paige Huffaker |
| A3 | Gallatin Rangers GSC Lady Rangers 10 |
0408-02AG2U-0051 | Jordan Sharp |
| A4 | Tennessee United Soccer Club TUSC 2010 Girls Blue |
0424-01AG2U-0041 | Ray Trotter |
| A5 | Blount United Soccer Club Blount United Soccer Club - G12U |
0201-01AG2U-0241 | Marco Browning |