| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy - 2010G Navy |
0316-029G2U-7846 | Don Maples |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 10G |
0324-02SJG2U-0028 | Casey Payne |
| A3 | Germantown Legends Germantown Lady Legends 2010 Black |
0606-01AG2U-0225 | Lance Loeffel |
| A5 | Kings Hammer MSC 2010 Lady Strikers Pool 1 |
0515-01DG2U-0129 | Serkan Orsan |
| A6 | Nashville United Soccer Academy - 01 NUSA G10 Maroon |
0534-01AG2U-0071 | n/a |
| A7 | Tennessee Soccer Club TSC Williamson 2011 Girls Academy Premier Blue - T |
0522-01AG2U-2092 | Sterling Nabours |