| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 03/04B Red |
0324-021B9U-0024 | James Lessentine |
| A2 | Nashville United Soccer Academy - 01 NUSA B03 Maroon |
0534-011B9U-0122 | John Sintic |
| A3 | Nashville United Soccer Academy - 01 NUSA B03/04 Navy |
0534-012B9U-0067 | Ethan Keogh |
| A4 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B19U Black |
0521-011B9U-0009 | Thomas Williams |
| A5 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B03 |
0213-01CB9U-0009 | Charles Perciful |
| A6 | Tri-Cities United Soccer Club Tri-Cities United 03/04 Boys Premier |
0106-011B9U-0010 | Ovidiu Chirica |
| A7 | Tennessee Soccer Club TSC Murfreesboro 2003 Boys Showcase |
0522-011B9U-2010 | Jorge Suarez |
| A9 | Tennessee Soccer Club TSC Williamson 2003/2004 Boys Showcase |
0522-012B9U-0147 | Christian Dale |
| A10 | Tennessee United Soccer Club TUSC 2003 Boys NPL |
0424-011B9U-0022 | Nathanael Hubert |