| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 05 Lobos Rush Premier |
0603-011B7U-0045 | Jason Haluska |
| A2 | Pateadores Soccer Club - Nashville Barca Academy 2005B Blue |
0500-01D1B7U-0007 | Javier Garcia |
| A3 | Kononia Soccer Club - 01 Kononia FC FIRE |
0611-011B7U-1975 | Dennis Headley |
| A4 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 05 Boys - SL |
0624-011B7U-2394 | William Pryor |
| A5 | Nashville United Soccer Academy - 01 NUSA B05 Maroon |
0534-011B7U-0051 | Robert Stewart |
| A6 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B17U Black |
0521-011B7U-0008 | Jun Taulbee |
| A7 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B05 |
0213-01CB7U-0008 | Scott Muir |
| A8 | Tennessee Soccer Club TSC Murfreesboro 2005 Boys Showcase |
0522-011B7U-0087 | Jason Finley |
| A9 | Tennessee Soccer Club TSC Williamson 2005 Boys Showcase |
0522-011B7U-0089 | James Pollock |