| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 06B Red |
0324-021B6U-0030 | Kuda Nhiwatiwa |
| A2 | Germantown Legends Germantown Legends 06 Black |
0606-011B6U-0206 | A Carbognani - DO NOT USE |
| A3 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 06 Boys Blue - SL |
0624-011B6U-2396 | Carl Schmitt |
| A4 | Nashville United Soccer Academy - 01 NUSA B06 Maroon |
0534-011B6U-0010 | Sunebari Ndam |
| A5 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B16U Black |
0521-011B6U-0007 | Jun Taulbee |
| A6 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B06 |
0213-01CB6U-0015 | Scott Muir |
| A7 | Tri-Cities United Soccer Club Tri-Cities United 06 Boys Premier |
0106-011B6U-0007 | Nicolas Boegemann |
| A8 | Tennessee Soccer Club TSC Murfreesboro 2006 Boys Showcase |
0522-011B6U-2065 | Jason Finley |
| A9 | Tennessee Soccer Club TSC Williamson 2006 Boys Showcase |
0522-011B6U-0138 | Tre Arhagba |
| A10 | Tennessee United Soccer Club TUSC 2006 Boys NPL |
0424-011B6U-0018 | Nate Sego |