| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Rhea County Football Club Play (RC3) Rc3 FC Rampage |
0322-01D3B4U-0025 | Jeremy Toliver |
| A2 | Tri-Cities United Soccer Club Tri-Cities United 08/09 Boys Premier |
0106-011B4U-0005 | Nicolas Boegemann |
| A3 | Emerald Force Soccer Club - 01 Emerald Force 08 Chelsea |
0214-013B4U-4585 | Elvyn Davidson |
| A4 | Chattanooga FC Academy Chattanooga FC Academy - 2008B Gray |
0316-023B4U-7816 | Michael Larson |
| A5 | Redoubt Soccer Association - 01 2008 Redoubt Generals |
0312-013B4U-0001 | Doug Allen |
| A6 | Tennessee United Soccer Club TUSC 2008 Boys Blue |
0424-013B4U-0026 | David Ingram |
| A7 | FC Alliance ChallengeTeams FC Alliance 2008B Showcase |
0204-02BB4U-0003 | Jevon Joseph |