| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Blount United Soccer Club Blount United Soccer Club - B13U |
0201-012B3U-0231 | Darren Sutton |
| A2 | Chattanooga FC Academy Chattanooga FC Academy - 2009B Navy |
0316-022B3U-7826 | Wesley Ford |
| A3 | FC Alliance ChallengeTeams FC Alliance 2010B Pre-ECNL |
0204-02SB2U-0177 | Steven Townley |
| A4 | FC Alliance ChallengeTeams FC Alliance 2009B Showcase |
0204-022B3U-0001 | Jevon Joseph |
| A5 | Knoxville FC (Crush) Knoxville FC Crush 09 B Premier |
0206-012B3U-0130 | Jorge Valdivia |
| A6 | Emerald Force Soccer Club - 01 Emerald Force 09 Manchester City |
0214-012B3U-1246 | Kelvin Scott |
| A7 | Johnson City Futbol Club - 01 JCFC 2009B |
0216-012B3U-0004 | Izudin Operta |