| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy - 2009B White |
0316-022B3U-7827 | Thomas Bentham |
| A2 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 09B Red |
0324-022B3U-0031 | Josue Fernandez |
| A3 | Kings Hammer MSC 09 Strikers Showcase |
0515-012B3U-0122 | Fifi Dadson |
| A4 | Nashville United Soccer Academy - 01 NUSA B09 Navy |
0534-012B3U-0061 | S Hughes |
| A5 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B13U Red |
0521-012B3U-0010 | Fulgence Letema |
| A6 | Tennessee United Soccer Club TUSC 2009 Boys Red |
0424-012B3U-0013 | Adolfo Lopez |
| A7 | Tennessee United Soccer Club TUSC 2010 Boys Red |
0424-01AB2U-0049 | A Morrell - DO NOT USE |