| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2008 Lobos Rush Premier |
0603-011B3U-0023 | David McAlister |
| A2 | Pateadores Soccer Club - Nashville Barca Academy Nashville 2008B Garnet |
0500-01D1B3U-0031 | Pablo Fuentes |
| A3 | Chattanooga FC Academy Chattanooga FC Academy 2008 Navy Boys |
0316-021B3U-7791 | Kristopher Ray |
| A4 | Germantown Legends Germantown Legends 08 Black |
0606-011B3U-0003 | John Adams |
| A5 | Knoxville Reds Football Club - 01 Knoxville Reds 08 Celtic |
0290-01D1B3U-0005 | Derrick Long |
| A6 | Tennessee Soccer Club TSC 2008 Boys ECNL |
0522-011B3U-0131 | Jordan Grant |
| A7 | Tennessee Soccer Club TSC 2008 Boys Nike |
0522-011B3U-0132 | Josh Severns |
| A8 | Tennessee Soccer Club TSC 2009 Boys Showcase Blue |
0522-011B3U-0044 | Michael Otto |
| A9 | Tennessee United Soccer Club TUSC 2008 Boys Red |
0424-011B3U-0031 | Nathanael Hubert |