| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC - 2012G Navy |
0316-029G1U-7878 | Scott Chapman |
| A2 | Clarksville Soccer Club Clarksville SC - G2012 Blue |
0422-01AG1U-0004 | Edward Brooks |
| A3 | Kings Hammer KHM 12G Premier I |
0515-01DG1U-0173 | Erich Delfs |
| A4 | Knoxville FC (Crush) Knoxville FC Crush 2012G Santa Clara |
0206-01CG1U-5565 | Victoria Weinhandl |
| A5 | Tennessee Soccer Club TSC Williamson 2012 Girls Academy United- JDL |
0522-01AG1U-2166 | Sterling Nabours |
| A6 | Wilson United Soccer Club WUSC - 2012 Girls Black |
0406-01AG1U-0015 | Justin Berry |