| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy - 2012G Navy |
0316-029G0U-7851 | Scott Chapman |
| A2 | Clarksville Soccer Club Clarksville SC - G2012 Maroon |
0422-01AG0U-0016 | Edward Brooks |
| A3 | Tennessee Soccer Club TSC Williamson 2012 Girls Academy Elite Blue - JDL |
0522-01AG0U-2102 | Danielle Padilla |
| A4 | Aris FC Aris FC - 2012G |
0530-02AG0U-0111 | Stephen Adedo |
| A5 | Knoxville FC (Crush) Knoxville FC Crush 2012G Santa Clara |
0206-01CG0U-5565 | Ashley Babjac |
| A6 | Tennessee Soccer Club TSC Williamson 2012 Girls Academy Premier White - |
0522-01AG0U-2101 | Danielle Padilla |